Name:Likui Zhang

Position:Associate Professor

Tel:13815810917

Email:lkzhang@yzu.edu.cn

Introduction:

Likui Zhang, Ph.D., Associate Professor, Master Supervisor, Deputy Director of the Department, National Natural Science Foundation System Review Expert, Ministry of Education Ph.D./Master Degree Thesis Review Expert, Instructor of Excellent Master Dissertation of Jiangsu Province, Instructor of Postgraduate Innovation Project of Jiangsu Province, Instructor of Science and Technology Innovation Project of University Students of Jiangsu Province, Director of Jiangsu Ocean Society, Young and Middle-aged Academic Leader of Yangzhou University, Winner of the First Prize of Yangzhou Outstanding Natural Science Academic Paper and Instructor of Excellent Bachelor Dissertation of Yangzhou University.

Currently, I have presided over the completion of 12 projects at the national, provincial, and municipal levels, and participated in 4 national and provincial projects. I have published 28 SCI papers, 12 Chinese core journal papers, and participated in writing 3 English books. I am acting as an Editor for international SCI journal Frontiers in Microbiology and EC Microbiology and as a Reviewer Expert in reviewing international SCI journals such as Frontiers in Microbiology, Extremophiles, and Journal of Structural Biology, and Chinese core journal such as Acta Microbiologica Sinica.

Research direction: Extreme Environmental Microbiology and Molecular Biology

Research projects: High temperature will increase the rates of base deamination and hydrolysis in DNA, which causes DNA damage. Generally, replication of damaged bases in DNA before they are repaired will lead to gene mutation. Since hyperthermophilic Archaea (HA) thrive in high-temperature environments, the stability and integrity of their genomic DNA are facing severe challenges posed by high temperatures. However, HA have a spontaneous mutation frequency similar to that of mesophilic microorganism E. coli, suggesting that HA have a more effective DNA repair pathway than E. coli to repair damaged DNA caused by high temperature. Currently, knowledge on DNA repair of HA is incomplete. Our laboratory is taking the hyperhermophilic Sulfolobus and Thermococcus as the research objects, and employing technical methods of molecular biology, biochemistry, and genetics to study DNA repair of HA. The projects currently under investigation are as follows:

1. Catalytic mechanism and physiological function of repairing damaged bases in DNA driven by archaeal NucS endonucleases

2. Catalytic mechanism and physiological function of repairing damaged bases in DNA driven by archaeal endonuclease V

3. Catalytic mechanism of A novel bi-functional uracil DNA glycosylase from HA

Education:

2010.06－2012.11 University of Alberta Postdoctoral fellow

2004.09－2010.05 Institute of Microbiology, Chinese Academy Sciences Ph.D

2007.11－2009.03 The OHIO State University Visiting student

2001.09－2004.07 Nanjing Agricultural University MSc

1997.09－2001.07 Anhui Agricultural University BSc

Achievements in scientific research:

Research fund

1. National Natural Science Foundation of China grant (No. 41306131)

2. Natural Science Foundation of Jiangsu Province (No. BK20191219)

3. Natural Science Foundation of Jiangsu Province (No. BK20130440) ‍

4. Natural Science Foundation for College and University of Jiangsu Province (No. 13KJB180029)

5. Open Project of State Key Laboratory of Microbial Resources (No. SKLMR-20130603)

6. Open Project of State Key Laboratory of Microbial Metabolism (No.MMLKF18-05)

7. Open Project of State Key Laboratory of Microbial Technology (No. M2016-09)

8. Open Project of State Key Laboratory of Virology (No. 2016KF006)

Representative publication (* corresponding author)

1. Zhang D, Pan Y, Zhao D, Yang Y, Zhu J*,Zhang L*, Yang Z*. Dissecting the effect of continuous cropping of potato on soil bacterial communities as revealed by high-throughput sequencing.Plos One, 2020, 15(5):e0233356.

2. Zhang L*, Jiang D, Wu M, Yang Z*, Oger P*. New insights into DNA repair revealed by NucS endonucleases from hyperthermophilic Archaea.Frontiers in Microbiology, 2020, in press.

3. Zhang L*, Jiang D, Shi H, Wu M, Gan Q, Yang Z*, Oger P*. Characterization and application of a family B DNA polymerase from the hyperthermophilic and radioresistant euryarchaeonThermococcus gammatolerans. International Journal of Biological Macromolecules. 2020, 156:217-224.

4. Gan Q, He M, Shi H, Yang Z*, Oger P*, Ran L,Zhang L*. Characterization of a Family IV uracil DNA glycosylase from the hyperthermophilic euryarchaeonThermococcus barophilus Ch5. International Journal of Biological Macromolecules, 2020, 146: 475-481.

5. Zhang L*, Shi H, Gan Q, Wang Y, Wu M, Yang Z*, Oger P*, Zheng J. An alternative pathway for repair of deaminated bases in DNA triggered by archaeal NucS endonuclease.DNA Repair (Amst), 2020, 85:102734.

6. Zhang L*, Li Y, Shi H, Zhang D, Yang Z*, Oger P*, Zheng J. Biochemical characterization and mutational studies of the 8-oxoguanine DNA glycosylase from the hyperthermophilic and radioresistant archaeonThermococcus gammatolerans. Applied Microbiology and Biotechnology, 2019, 103(19):8021-8033.

7. Shi H, Gan Q, Jiang D, Wu Y, Yin Y, Hou H, Chen H, Xu Y, Miao L, Yang Z*, Oger P*,Zhang L*. Biochemical characterization and mutational studies of a thermostable uracil DNA glycosylase from the hyperthermophilic euryarchaeonThermococcus barophilus Ch5.International Journal of Biological Macromolecules, 2019, 134:846-855.

8. Shi H, Huang Y, Gan Q, Rui M, Chen H, Tu C, Yang Z*, Oger P*,Zhang L*. Biochemical characterization of a thermostable DNA ligase from the hyperthermophilic euryarchaeonThermococcus barophilus Ch5.Applied Microbiology and Biotechnology, 2019, 103(9):3795-3806.

9. Li Y, Zhu X, Zhang W*, Zhu D*, Zhou X,Zhang L*. Archaeal communities in the deep-sea sediments of the South China Sea revealed by Illumina high-throughput sequencing.Annals of Microbiology, 2019, 69:8.39-848.

10. Wang Y,Zhang L*, Zhu X, Li Y, Shi H, Oger P, Yang Z. Biochemical characterization of a thermostable endonuclease V from the hyperthermophilic euryarchaeonThermococcus barophilus Ch5. , 2018, 117:17-24.

11. Zhang L*. New insights into DNA polymerase function revealed by phosphonoacetic acid-sensitive T4 DNA polymerases.Chemical Research in Toxicology, 2017, 30(11):1984-1992.

12. Huang Y, Zhang L*. An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology. Methods in Molecular Biology, 2017, 1498:375-383.

13. Zhang L*, Xu D, Huang Y, Zhu X, Rui M, Wan T, Zheng X, Shen Y, Chen X, Ma K, Gong Y*. Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease.Scientific Reports, 2017, 7:42542.

14. Zhang L*, Kang M, Xu J, Xu J, Shuai Y, Zhou X, Yang Z*, Ma K. Bacterial and archaeal communities in the deep-sea sediments of inactive hydrothermal vents in the Southwest India Ridge.Scientific Reports, 2016, 6:25982.

15. Zhang L*, Huang Y, Xu D, Yang L, Qian K, Chang G, Gong Y*, Zhou X, Ma K. Biochemical characterization of a thermostable HNH endonuclease from deep-sea thermophilic bacteriophage GVE2.Applied Microbiology and Biotechnology, 2016, 100(18):8003-12.

16. Zhang L*, Kang M, Huang Y, Yang L. Fungal communities from the calcareous deep-sea sediments in the Southwest India Ridge revealed by Illumina sequencing technology.World Journal of Microbiology and Biotechnology, 2016, 32(5):78.

17. Zhang L*, Kang M, Xu J, Huang Y. Archaeal DNA polymerases in biotechnology.Applied Microbiology and Biotechnology, 2015, 99(16):6585-97.

18. Zhang L, Radziwon A, Reha-Krantz LJ*, Targeted Mutagenesis of a Specific Gene. Methods in Molecular Biology. 2014, 1163: 109-129.

19. Wu Y, Chen H,Zhang L*. Research progress of 8oxoG DNA glycosylase from hyperthermophilic Archaea.Acta Microbiologica Sinica, 2020, in press. (In Chinese)

20. Gan Q, Jiang D,Zhang L*. Research progress of uracil DNA glycosylase from hyperthermophilic Archaea.Acta Microbiologica Sinica, 2020, 60(2): 227-236. (In Chinese)

21. Li Y, Shi H,Zhang L*. Research progress of hyperthermophilic archaeal DNA repair endonucleases.Acta Microbiologica Sinica, 2019, 59(10):1889-1896. (In Chinese)

22. Huang Y, Qian K, Ping S, Jiang Z, Jiang W,Zhang L*, Yang Z*. Identification of amesophilic α-amylase-producing strain isolated from the deep-sea sediment and enzymatic characteristics.Food Industry Technology, 2018, 39(04):111-116+130. (In Chinese)